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Image Search Results
Journal: International Journal of Molecular Medicine
Article Title: Quercetin inhibits NTHi-triggered CXCR4 activation through suppressing IKKα/NF-κB and MAPK signaling pathways in otitis media
doi: 10.3892/ijmm.2018.3577
Figure Lengend Snippet: Chemokine CXC receptor 4 (CXCR4) is activated by nontypeable Haemophilus influenzae (NTHi) in epithelial cells of middle ear. (A) NTHi was used to treat human middle ear epithelial cells (HMEECs) (25, 50 and 250 for MOI) for 6 h, followed by measurement of CXCR4 gene levels through RT-qPCR analysis. (B) CXCR4 mRNA levels were detected via RT-qPCR analysis in NTHi (MOI of 50)-treated HMEECs cells for different times as indicated. (C) HMEECs were exposed to NTHi for 6 h, and CXCR4 expression from protein levels was evaluated through enzyme-linked immunosorbent assay (ELISA) method. (D) The renal epithelial cells (HK2), (E) liver epithelial L02 cells, and (F) human lung epithelial cells (BEAS-2B), as well as (G) mouse lung epithelial cells (MLE-12) were treated with NTHi for different times, and the CXCR4 mRNA levels were tested through RT-qPCR assays. (H) HMEECs were exposed to various strains of NTHi (2019, 12 and 2866) for 6 h. RT-qPCR analysis was applied to explore CXCR4 gene levels. (I) The experimental animals were inoculated with NTHi for various times as indicated, and CXCR4 mRNA levels were calculated in the the middle ear tissue samples through RT-qPCR assays. The representative data are shown as SEM *** p<0.001 vs. the control group without any treatments.
Article Snippet: The
Techniques: Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay, Control